Useful Helicobacter pylori Antibody for ELISA Testing

IMCB
NTHU
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Dako

Product Name:

Polyclonal Rabbit Anti-Helicobacter Pylori

Catalog Number:

B0471

Used this antibody to generate a method for ELISA testing and is very useful. In summary, the results show the changes in the total amount of H. pylori being internalized by the cells.

Experimental Design and Results Summary

Applications

ELISA

Sample

Gastric cancer cell line AGS cell lysate

Primary Incubation

H pylori Antibody

Blocking Agent

0.5% BSA

Secondary Incubation

Anti Rb linked antibody linked with HRP

Tertiary Incubation

TMB Substrate

Detection

OD450

Results Summary

The results detected is very accurate and shows the changes in the total amount of H. pylori being internalized by the cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very specific and easy to administer

The Bad

Multiple washing steps required due to its high sensitivity.

The Bottom Line

Recommended for those whom are studying H pylori pathogenicity.

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