Flow Cytometry Anti-Human CD4 Antibody in PE

Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-human CD4 Antibody

Catalog Number:

357403

One facet of the research in our lab is examining human immune responses of human PBMCs transplanted into immunodeficient mice. This requires the use of flow cytometry to detect specific cell populations isolated from mouse tissue.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen with transplanted human PBMCs

Primary Incubation

20 minutes on ice

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Detection of human CD4+ cell populations

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains desired antigen when used at optimal dilution

The Bad

None

The Bottom Line

Great antibody for detecting human CD4+ cell populations

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