Good Antibody To Pair With PECy7-CD45.1 To Track The Donor Cells After Adoptive Transfer

October 03, 2018

Immunology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC-CD45.2

Catalog Number:

109806

CD45.1 and CD45.2 are most commonly used congenic markers in mice. Cells expressing CD45.1 (eg. WT) or CD45.2(eg. KO) can be distinguished by antibodies to CD45.1 and CD45.2. These cells can be tracked after adoptively transferred to the host mice that express both CD45.1 and CD45.2 (see image). After testing different combinations of CD45.1 and CD45.2 antibodies, we found that this FITC-CD45.2 antibody is good to pair with PECy7-CD45.1.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 15-20 minutes on ice

Blocking Agent

2% FBS in PBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS

Results Summary

Good separation of CD45.1+, CD45.2+, and CD45.1+CD45.2+ cells

DOI or PMID #

29386256

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good antibody to use and pair with PECy7-CD45.1

The Bad

NA

The Bottom Line

These are good antibodies to congenial markers, which allow the donor cells to be distinguished from the endogenous host cell populations. It is a useful tool for studying immune systems.

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