A Rabbit Polyclonal mCherry Antibody That Can Label tdTomato

September 26, 2018

Institute of Biotechnology
National Tsing Hua University
Ph.D. Student

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Company:

Genetex

Product Name:

mCherry antibody

Catalog Number:

GTX128508

We used this rabbit polyclonal mCherry antibody to label tdTomato (Trans-TANGO technique) in the Drosophila eye system (R8 photoreceptors)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Drosophila Brain

Primary Incubation

1:100 4 degree overnight

Blocking Agent

5% normal goat serum in 0.3%PBST

Secondary Incubation

1:1000 in RT for 4hrs

Tertiary Incubation

N/A

Detection

Cy3 (543 nm) Immunofluorescence

Results Summary

We use this antibody to labeled tdTomato (Trans-TANGO) in Drosophila brain driving by Pan-R8-Gal4 to express in R8 photoreceptors.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive and clean

The Bad

Little background

The Bottom Line

This antibody is sensitive and with little background.

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