Anti-PCNA for Paraffin Embedded Samples

September 25, 2018

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-PCNA

Catalog Number:

ab152112

This antibody was used to detect Proliferating Cell Nuclear Antigen, to quantify the number of actively dividing cells during wound healing in a murine full excision skin wound healing model.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse skin

Primary Incubation

Overnight, 1:1000, 4 degrees

Blocking Agent

5%NGS/1xPBS-0.1%TritonX100 at RT for 60’

Secondary Incubation

Rabbit biotinylated antibody 1:200

Tertiary Incubation

-

Detection

DAB Kit (Vector Cat SK-4100)

Results Summary

The nuclei of actively dividing cells were clearly stained and manually quantified at 20x magnification

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean staining

The Bad

Requires an antigen unmasking step

The Bottom Line

A good antibody to detect PCNA in paraffin embedded samples

Share your experience with other scientists. Write a Review! »

Join the discussion