Great PDL1 Antibody for Western Blot

September 19, 2018

Dentistry
National Defense Medical Center
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

PDL1

Catalog Number:

GTX104763

We study the interactions between hypoxia and inflammation in human oral cancer cells. This antibody was used to detect PDL1 expression in the SAS cell line by Western blot. Signal is strong.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Tongue cancer cell

Primary Incubation

1:500 dilution overnight in blocking resgent

Blocking Agent

3%BSA in blocking resgent

Secondary Incubation

1:5000 dilution overnight in blocking resgent

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

Various whole cell were separated by 10% SDS-PAGE, and the membrane was blotted with PD-L1 antibody (GTX104763) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

high sensitivity

The Bad

None

The Bottom Line

We will continue to use this antibody

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