BD Bioscences
Purified Mouse Anti-Tom20
612278
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Browse Similar Products: TOM20 Immunofluorescence Antibodies
My experiment was focused on evaluating the cellular localisation of a transporter. I used the anti-Tom20 antibody as a marker of mitochondria in HEK293 cells transiently transfected with a MYC-fused isoform of the transporter. This antibody was used for immunofluorescence on HEK293 cells fixed with paraformaldehyde 4%, 48 hours after transfection.
Immunofluorescence
HEK293 cell line
Antibody diluted 1:200, incubated for 1 hour at RT
BSA 2%
Dylight 549 goat anti-mouse secondary antibody (115-505-174), incubated 50 minutes at RT
N/A
Olympus FluoView FV1000
The antibody performs quite well at high dilutions on fixed cells. It could be optimised raising the concentration and/or extending the incubation time.
Works in immunofluorescence on cells
Not always very defined staining
OK as mitochondrial marker