Good Antibody for T Cell Activation, Paired With PE-CD62L

Immunology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC-CD44

Catalog Number:

103012

We use a mouse model to study CD4 and CD8 T cell signaling and activation. We used PE-CD62L and APC-CD44 antibodies to detect CD4 and CD8 T cell activation status after listeria infection.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:100, 15-20 minutes on ice

Blocking Agent

FBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS

Results Summary

Nice separation for CD44+ and CD44+, Together with PE-CD62L, naive, activated, or effect/memory T cell populations can be detected.

DOI or PMID #

29386256

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong and clean antibody

The Bad

NA

The Bottom Line

Good antibody to use.

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