Human Anti-CD20-APC-Vio770 Antibody

September 14, 2018

Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-CD20 APC-Vio770

Catalog Number:

130-108-292

This anti-CD20 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). We are particularly interested in the innate compartment of lymphocytes, and use bright CD20 and CD19 recognizing antibodies to identify and exclude B lymphocytes. The Anti-CD20-APC-Vio770 human antibody from Miltenyi Biotec is a mouse monoclonal antibody to LT20 to CD20.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human PBMCs

Primary Incubation

1:20, 37°c for 15min

Blocking Agent

PBS/BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

CD20 staining is clear with this antibody at the suggested titration 1:20 on PBMCs

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clear, bright antibody

The Bad

N/A

The Bottom Line

Clear, reliable and bright antibody for CD20 expressing cells.

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