EMD Millipore
Benzonase nuclease
70746-3
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Separation of protein on chromatography columns from proteins extracted from gram negative bacteria treated with 1X BugBuster lysis reagent with Benzonase nuclease at 25 units/ml lysis reagent. Flow through the columns were efficient and got good yields of proteins.
Extraction of proteins from gram negative bacterial cells
Gram negative bacterial cell pellets (from 5 ml culture) treated with 1X Novagen BugBuster lysis reagent
I have used Novagen BugBuster protein extraction kit for extracting proteins. Followed protocol given in the kit's handbook. Used Benzonase nuclease with 1X Bugbuster lysis reagent at 25 units/ml. Then followed remaining steps for final separation of proteins using size exclusion column chromatography.
Dilute the benzonase nuclease enzyme in 50% glycerol if required to reduce the concentration.
After treating protein extracts with Benzonase nuclease, the viscosity was reduced to greater extent and flow through the chromatography columns were efficient.
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Easy to use and simple and efficient for removal contaminating nucleic acids.
Bit expensive.
Ideal enzyme for protein preps and especially protein extracts to be separated on chromatographic columns.