Extraction of High Amounts of Plasmid

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Pharmaceutical Technology
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Company:

Qiagen

Product Name:

EndoFree Plasmid Maxi Kit (10)

Catalog Number:

12362

We use this kit for extraction of high amounts of plasmid from Escherichia coli. Since we use this plamids in non viral gene therapy and inject the final formulations, we require the endo free conditions to avoid immune reactions in the in vivo experiments.

Experimental Design and Results Summary

Application

Plasmid DNA extracton

Starting Material

Escherichia coli containing the plasmids after transfection

Protocol Overview

It is easy to follow the manufacturer´s recommendations. Follow all the steps carefully and good results are obtained.

Tips

Follow carefully manufacturer recommendaitons

Results Summary

Each extraction can provide up to 500 microgrames of plasmid DNA.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy to perform the extraction with this kit

The Bad

none

The Bottom Line

Plasmid DNA is extracted at high amounts and good purity for gene therapy studies

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