Biolegend
APC anti-mouse CD183 (CXCR3) Antibody
126511
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Our goal was to analyze the response different cell types in mouse lungs. Mouse lungs were digested with collagenase, strained, and stained for flow cytometry with a cocktail of different antibodies. The samples were analyzed on a BD LSRII flow cytometry. The FACS plot shown is after gating on Single, Live, CD45+, TCRb+ cells.
Flow Cytometry
Collagenase-digested mouse lung tissue
1:200 for 20 minutes on ice
24G2 in 3% BSA in PBS for 10 minutes on ice prior to staining in 3% BSA
None
Flow cytometer (BD LSRII)
We were able to detect a specific, clear population of Cxcr3+ cells within the TCRb+. However, the staining is not particularly bright, so a brighter fluorophore may be useful for this marker.
N/A
Specific and clear positive population
Not particularly bright
If you must use the APC channel, this antibody will suffice, however would recommend a brighter fluorophore for some applications.