Biolegend
Keratin 5 Polyclonal Antibody, Purified
905501
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Browse Similar Products: Keratin 5 Immunofluorescence Antibodies
Our laboratory studies T Cell Acute Lymphoblastic Leukemia (T-ALL), a cancer of developing T cells. In order to examine the composition and organization of the tumor microenvironment, our lab utilizes a wide variety of markers to identify specific cellular subsets of the tumor microenvironment. We picked this antibody for immunofluorescence imaging knowing that it would characterize the architecture of the tumor microenvironment in T-ALL in our mouse model. Keratin 5 (K5) is a marker of medullary thymic epithelial cells (mTECs), so I used this antibody to examine the architecture of the thymic medulla with IF imaging.
Immunofluorescence
Thymus
1:200 room temp for 1 hour
TNB blocking buffer, perkin elmer
anti rabbit AF 594, 1 hr at room temperature
None
DMi8 Leica Microscope
This is a super bright stain and it works great for me.
Not yet published
N/A
This works great on thymus medulla and is a very standard stain in our lab.
K5 is great for IF imaging, but there are better antibodies for flow cytometry.
I love it and it is fantastic at defining thymic medulla.