Promega
CytoTox-ONE™ Homogeneous Membrane Integrity Assay
G7891
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We selected this kit to measure the release of Lactate DeHydrogenase in medium to easily check the viability of cell cultures and organotypic brain cultures.
Viability test measured by fluorescence
Culture media
The kit allows us to measure membrane leakage of cells and organotypic cultures exposed to toxins.It is important to set up correctly the temperature of the test as suggested by the company because, on the contrary, the results could change. Pay attention to drugs/toxins that could interfere with the fluorescence.
Temperature and possible interference with fluorescence
The kit gave us good values in term of fluorescence, with high repeatability.
10.1038/srep41032
N/A
Sensitive
Not easy to optimize
Good kit with high sensitivity, but it needs attention to optimization and possible interference.