Thermofisher
Streptavidin, Alexa Fluor™ 647 conjugate
S-21374
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Our laboratory studies T Cell Acute Lymphoblastic Leukemia (T-ALL), a cancer of developing T cells. In order to examine the composition and organization of the tumor microenvironment, our lab utilizes a wide variety of markers to identify specific cellular subsets of the tumor microenvironment. We picked this antibody for immunofluorescence imaging knowing that it would characterize the architecture of the tumor microenvironment in T-ALL in our mouse model. I aimed to use this as a secondary for a biotinylated primary antibody in order to detect dendritic cells in splenic tissue.
Immunofluorescence
Spleen
Anti-CD11c, NF18, biolegend
TNB blocking buffer, perkin elmer
Step 647, 1 hr at room temperature
N/A
DMi8 Leica Microscope
The main issue with this product is that it clumped and failed to completely dissolve. This left me with globs of color on my tissue. To correct this, I had to warm up the antibody, votex it, and centrifuge my antibody dilution for 1 min at 14000 rpm before each use.
Not yet published
Very bright and worked fine once optimized
It was annoying for the product to clump as it did
Works fine, but if you have any issues then you should optimize carefully.