Abcam
Rat TNF alpha ELISA Kit
ab100785
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I utilized this ELISA kit for the quantification of the TNFa alpha released in the culture media of rat organotypic hippocampal slice exposed to neurotoxic compounds. I selected this product because of its high range of concentration of measurable cytokine.
ELISA
Culture media from organotypic hippocampal cultures
I followed the protocol as described by the company. I paid attention to the washing steps that are always critical in ELISA kits.
Follow the kit instruction and wash well when requested
The kit works well in culture media. The kit was able to quantify TNF alpha in the control ( about 15 pg/mL) and in the treatment (about 2400 pg/mL) in the work
DOI:10.1038/s41598-018-25684-2
N/A
Easy to use
None
The product allows me to easily analyze the sample with high sensitivity.