Millipore
Anti-Integrin beta1 Antibody, activated, clone HUTS-4
MAB2079
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Integrins are cell surface transmembrane molecules that primarily mediate adhesion of cells to matrix proteins and induce cell signaling. Among integrins, beta 1 integrins play crucial roles in cell communications and migration of normal and cancer cells. Beta 1 integrins are also involved in stromal invasion and distant metastasis of cancer cells. We analyzed the levels of beta 1 integrin expression in human prostate carcinoma cells exhibiting different dissemination potentials. To achieve this goal, we compared several anti-integrin beta 1 antibodies, including HUTS-4, for their ability to recognize beta 1 integrin in western blotting under reducing and non-reducing conditions.
Western Blot
Lysates of human tumor cells; non-reducing conditions-20 microgram per lane; reducing conditions-40 microgram per lane
Antibody at a final concentration of 1 microgram/ml, ON incubation with gentle rocking in a cold room
5% milk in PBS-0.05% Tween 20
HRP-conjugated anti-mouse antibody at 1:5,000 in 5% milk in PBS-0.05% Tween 20
N/A
West Pico
The mAb HUTS-4 specifically detected one protein band with an apparent mol. wt. of ~130 kDa, which is expected for human beta 1 integrin. The high specificity of this antibody was confirmed by the lack of any positive signal from the cells in which beta 1 integrin was stably knocked down with beta 1-specific shRNA (beta 1-KD) compared with the signal intensity in control cells (Ctrl). Consistent with the description provided by the company, the HUTS-4 antibody detects beta 1 integrin only under non-reducing conditions as no positive band was detected in control cells under reducing conditions (Red).
The mAb HUTS-4 is known to detect only activated beta 1 integrin. In our western blot analyzes we used the cells cultured under conventional conditions, i.e. in cell culture medium supplemented with 5% FBS. ting conditions.
The antibody appears highly specific and sensitive.
None detected.
The antibody HUTS-4 is a sensitive mouse monoclonal antibody that specifically recognizes human beta 1 integrin in western blotting under non-reducing conditions.