Anti-Human CD203c (E-NPP3) Antibody for Multicolor Panel

August 27, 2018

JvGCRC
Nottingham Trent University
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Dazzle™ 594 anti-human CD203c (E-NPP3) Antibody

Catalog Number:

324624

This antibody has been used in optimisations of flow cytometry-based immunophenotyping of whole blood samples. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations and tested to ensure appropriate compensation was possible. CD203c was used in a nine colour staining panel to help identify mast cell progenitors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood

Primary Incubation

2.5uL PE/Dazzle™ 594-CD203c for 30 minutes at 4oC

Blocking Agent

FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Beckman Coulter Galios, Kaluza software

Results Summary

Clear staining that, in combination with FcεRIα expression, helped in the identification of mast cell progenitors. Prior to analysing erythrocytes were lysed using 1.5ml PharmLyse (BD, Cat# 555899) at room temperature for 30 minutes. This antibody fit well into a nine colour panel with no compensation issues.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Clear staining and useful for FACS analysis. Tritrations showed it was possible to reduce the volume suggested by the manufacturer.

The Bad

The antibody has only been tested for flow cytometry

The Bottom Line

Easy to use antibody pack that provided clear and distinct staining to help identify mast cell progenitors.

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