Great Antibody to Identify Donor T cells Using CD45.1

Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. In order to study this process, we perform a lot of adoptive transfer experiments of donor transgenic T cells (CD45.1 or others) into different CD90.2/CD45.2 WT B6 congenic hosts. In order to identify the donor T cells, we use CD45.1 to distinguish host versus donor especially in co adoptive studies of two donor cells into the same host. This marker is quite reliable since it separates quite well from the host but it is somewhat on the dimmer side. The PerCp Cy5.5 CD45.1 antibody fits nicely into certain panels since it's usually a dim channel compared to other brighter channels; however, this antibody is reliable although at a higher dilution. We were able to readily identify the donor cells using this antibody from the host cells.