ebioscience/Thermo Fisher
FOXP3 Monoclonal Antibody (FJK-16s), FITC, eBioscience
11-5773-82
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: FOXP3 Flow Cytometry Antibodies
Our lab studies the mTOR signaling pathway in T cell differentiation with an emphasis on both CD4 and CD8 T cells. We routinely conduct flow cytometry experiments to analyze the differentiation process into subsets based on transcription factor (TF) analysis. In order to look at Tregs, we analyze T cells for the expression of Foxp3, which is a canonical transcription factor. This antibody color and clone is quite reliable to readily identify Treg cells from a heterogeneous population such as from a spleen.
Flow Cytometry
Murine plenocytes
Intracellular Staining with eBio Fix Perm Kit. 1:200 dilution for 45 minutes at room temperatue
None
Flow cytometer
We are easily able to determine the Tregs from a spleen based on CD4+Foxp3+ T cells via flow analysis.
25849206
Make sure you use the Foxp3 transcription factor kit for staining since it is a transcription factor.
The signal was very strong and was able to identify population.
Higher dilution needed to see the difference so a bit costlier.
Great antibody and clone to determine Treg population via flow cytometry.