Excellent Antibody for CD205 Staining

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD205 (DEC-205) Antibody

Catalog Number:

359204

In this project the phenotyping of immune cells in murine skin and lymph node was investigated. The immune cells were attracted and activated by injected extracellular vesicles from mature murine dendritic cells. This antibody was used to study the CD205-expression in murine tissue by direct immunfluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Murine spleen

Primary Incubation

1:20; RT; 60 min

Blocking Agent

5 % FBS; RT, 30 min

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results!

DOI or PMID #

none

Additional Notes

none

Related Categories

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Summary

The Good

Specific signals, no background, easy to use!

The Bad

none

The Bottom Line

Will buy and use it again. Recommendable!

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