Great Antibody to Identify CD8 T Cells

August 08, 2018

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 650™ anti-mouse CD8a Antibody

Catalog Number:

100742

We are an immunology lab studying CD8 T cell memory differentiation. CD8 staining is routinely used to identify CD8 T cells during flow analysis. We performed adoptive transfer in vivo experiments, and harvest spleens during the acute response of infection. I used this antibody in my T cell panel to identify the CD8 T cells in the spleen. I chose this product since it fit well in my 12 color panel. Biolegend was one of the few vendors reliably offering this marker with this particular clone and color.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

1:500, 30 minutes, 4 deg in 2% FCS/PBS

Blocking Agent

FC block cells before surface panel staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometer

Results Summary

I analyzed the adoptively transferred cells during the acute response. However, I used this antibody first to get on the total CD8 T cells within the spleen before gating further.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very robust and bright antibody to identify donor cells.

The Bad

Please make sure to titrate the antibody before use since it can be quite bright depending on analyzer.

The Bottom Line

Great antibody to really identify and gate out CD8 T cells within samples for flow analysis.

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