Hot Start Taq DNA Polymerase with MgCl2

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

JumpStart™ Taq DNA Polymerase with MgCl2

Catalog Number:

D9307

We work with different transgenic mice that have up to three transgenes and identification of each of them via PCR amplification is crucial. One of the mouse models contains a copy of HIV virus, and gag detection can be tricky, often resulting in primer dimers in addition to non-specific amplification. Utilization og this kit helps reduce non-specific interactions during PCR amplification.

Experimental Design and Results Summary

Application

PCR

Starting Material

Genomic DNA purified from mouse tails

Protocol Overview

We follow our standard PCR protocol

Tips

None

Results Summary

Works great

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Work great

The Bad

None

The Bottom Line

Highly suggested with tricky PCRs

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