BioLegend
APC Annexin V
640920
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Browse Similar Products: Annexin V for Cell Viability and Proliferation
We often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the APC conjugated (versus FITC) reagent since it allows us to use several other antibodies which are only available as a FITC conjugate. We include a positive control for each assay, which is usually cells treated for 2-4 hours with hydrogen peroxide to induce cell death.
Flow Cytometry
K562 cells treated for 2 hours with 0.8mM hydrogen peroxide
15 minutes at room temperature
Miltenyi Human Fc block, 20uL per 10^7 cells
none
ThermoFisher Attune NXT
K562 cells were treated with hydrogen peroxide to induce cell death (blue) and compared with cells incubated with an APC isotype control (red).
N/A
Works as expected
None
Since BioLegend's Annexin V APC works as well as other competitors and is a lower price, I will continue to order from BioLegend when I need Annexin V.