Very Good Antibody to Detect CD90.1 Mouse T Cells

July 16, 2018

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Catalog Number:

202528

Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. In order to study this process, we perform a lot of adoptive transfer experiments of donor transgenic T cells (CD90.1) into different WT B6 congenic hosts (CD90.2). In order to identify the donor T cells, we use CD90.1 to distinguish host versus donor. This marker is quite reliable since it separates quite well from the host. The AF700 Thy1.1 antibody fits nicely into certain panels since it's usually a dim channel compared to other brighter channels; however, this antibody is quite bright. We were able to readily identify the donor cells using this robust and bright antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Splenocytes

Primary Incubation

1:2000, 30 minutes, 4 deg in 2% FCS/PBS

Blocking Agent

FC block cells before surface panel staining

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometer

Results Summary

I analyzed the adoptively transferred cells (CD90.1) during the acute response of an adoptive transfer experiment.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very robust and bright antibody to identify donor cells.

The Bad

Please make sure to titrate the antibody before use since it can be quite bright.

The Bottom Line

Truly a great antibody to fit into any multi-parameter flow panels. Since AF700 is usually on the brighter side, this antibody fits nicely into the panel to look at more important markers from the donor cells.

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