Biolegend
Brilliant Violet 510™ anti-mouse CD90.2 Antibody
105335
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Browse Similar Products: CD90.2 Flow Cytometry Antibodies
Our lab studies the role of mTOR signaling in CD8 T cell differentiation and function. In order to study this process, we perform a lot of adoptive transfer experiments of donor transgenic T cells (CD90.2) into different CD90.1 WT B6 congenic hosts. In order to identify the donor T cells, we use CD90.2 to distinguish host versus donor especially in co adoptive studies of two donor cells into the same host. This marker is quite reliable since it separates quite well from the host but it is somewhat on the dimmer side. The BV510 Thy1.2 antibody fits nicely into certain panels since it's usually a dim channel compared to other brighter channels; however, this antibody is reliable. We were able to readily identify the donor cells using this antibody from the host cells.
Flow Cytometry
Murine T cells
1:300. 30 mins, 4 deg incubation
FC blocking in FACS buffer (2% FBS, .2% NaAzide/PBS)
None
Flow cytometer
We are able to identify the donor T cells from the host based on CD90.2 expression with CD8. We further analyzed this population after identification.
N/A
The signal was very strong and was able to identify population.
Just higher dilution of antibody is needed.
Not the most ideal antibody to use in this channel, but it gets the job done to identify donor T cells.