Anti-Mouse CD8a Antibody for PBMCs

July 16, 2018

Virology and Immunology
The J. David Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen™

Product Name:

PerCP-Cy™5.5 Rat Anti-Mouse CD8a

Catalog Number:

551162

Our lab works to understand how protective innate and adaptive immune responses are elicited and maintained. We use this antibody to see CD8 positive cells. We picked this antibody knowing it's reliable and strong.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PBMCs

Primary Incubation

4 C for 30 minutes at 4:300 dilution

Blocking Agent

FC block (1:1000)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Our flow cytometer was able to see CD8 positive cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody

The Bad

None

The Bottom Line

It's a good antibody that's showing up well on our flow cytometer. I will continue to use it.

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