Strong CD45.2 Antibody for Mouse PBMCs

Virology and Immunology
The J. David Gladstone Institutes
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen™

Product Name:

FITC Mouse Anti-Mouse CD45.2

Catalog Number:

561874

Our lab works to understand how protective innate and adaptive immune responses are elicited and maintained. We use this antibody to discern the congenic marker of cells. Mice showing a certain congenic marker is then used for an experiment or for breeding. We picked this antibody knowing it's reliable and strong.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PBMCs

Primary Incubation

4 C for 30 minutes at 3:300 dilution

Blocking Agent

FC block (1:1000)

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Our flow cytometer was able to discern CD45.2 positive cells in mice blood samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody

The Bad

None!

The Bottom Line

It's a good antibody and I will continue to use this antibody.

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