V5 Tag Antibody Used in a N2a Cell Line

August 14, 2018

Institute of Biotechnology
National Tsing Hua University
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Genetex

Product Name:

V5 tag antibody

Catalog Number:

GTX82563

We used N2a cell line and transfected it with our overexpressing target construct. The construct contains a V5 fusion tag so we used this antibody to label the expression level of the construct.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

N2a Cell line

Primary Incubation

1:100 4 degree 1 hr

Blocking Agent

5% normal goat serum in 0.3%PBST

Secondary Incubation

1:1000 in RT for 1hr

Tertiary Incubation

N/A

Detection

Cy5 (633nm) immunofluorescence

Results Summary

We transfected DNA modified construct to N2a cell which is fusion with a v5 tag, use this antibody can easily detect our target protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive in cells

The Bad

little background, doesn't work in Drosophila Brain

The Bottom Line

This antibody is unstable in Drosophila Brain immunostaining, it work sometimes, but seldom failed under the same condition.

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