Sensitive mCherry Antibody in Drosophila Brain

August 07, 2018

Institute of Biotechnology
National Tsing Hua University
Ph.D. Student

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Company:

Genetex

Product Name:

mCherry antibody [GT857]

Catalog Number:

GTX630189

We use this antibody to detect mCherry that expressing in Drosophila's visual system which is drive by Gal4 driver that specific appear in R8 photoreceptor.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Drosophila Brain

Primary Incubation

1:200 4 degree overnight

Blocking Agent

5% normal goat serum in 0.3%PBST

Secondary Incubation

1:1000 in RT for 4hrs

Tertiary Incubation

N/A

Detection

Cy3 (543nm) immunofluorescence

Results Summary

We use this antibody to detect mCherry signals which is expressing in Drosophila's trans-medulla TM5c neuron.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Sensitive and clean

The Bad

little background

The Bottom Line

This antibody is very sensitive that we can see Tm5c neuron's processes in medulla of Drosophila

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