Great Iba1 Immunofluorescence Antibody

July 20, 2018

NHRI (Institute of Cellular and System Medicine)
National Health Research Institute
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

Iba1 Antibody

Catalog Number:

GTX101495

This antibody was used to detect Iba1 expression in brain microvascular endothelial cells by immunofluorescence analysis. This antibody is great for staining microglia.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:100 dilution overnight at 4 degree in blocking reagent

Blocking Agent

PBS with 0.05% Triton X-100, 5% goat serum

Secondary Incubation

1:1000 dilution 1h at RT in blocking reagent

Tertiary Incubation

DAPI in PBS for 5min

Detection

Alexa Fluor 594 dye

Results Summary

This antibody perfectly detected Iba1 expression by IF in mouse brain tissues

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

We will continue to use this antibody

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