Anti Mouse CD27 Antibody

June 22, 2018

Immunology
Gladstone Institutes
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

PE-Cy™7 Hamster Anti-Mouse CD27

Catalog Number:

563604

Our research is focused on enhancing CD8+ T cell functionality for immunotherapeutic purposes. We were interested in delineating memory subsets of CD8+ P14 transgenic T cells based on CD27 expression. This antibody is very reliable and provides robust data by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse Splenocytes

Primary Incubation

30 minutes @ 4 degrees

Blocking Agent

CD16/32

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry using LSRII

Results Summary

The LG.3A10 clone works very well for detection of CD8+ T cell subsets based on CD27 and CD43.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable antibody that always works to clearly delineate subsets of CD8+ T cells

The Bad

N/A

The Bottom Line

Reliable antibody that always works to clearly delineate subsets of CD8+ T cells

Share your experience with other scientists. Write a Review! »

Join the discussion