Thermo Fisher Scientific
IFN gamma Mouse Uncoated ELISA Kit with Plates
88-7314-22
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mTORC1 activity very critical for the generation of effector CD8 T cells. Our lab studies the asymmetric division of mTORC1 activity during CD8 T cell differentiation. We used this ELISA to kit to assess effector function via IFNg production from supernatant from sorted CD8 T cells. We had very few cells yet we were able to detect reliable levels of IFNg production using this kit.
ELISA
Murine T cells
We coated our own strips with the capture antibody since we do not always use full plates otherwise we follow the protocol step-by-step.
Coat the plates overnight at 4 deg to ensure efficient coating of capture antibody.
We were able to detect differences in IFNg production from our experimental groups. The standard curve is very reliable.
27064374
N/A
Easy to use protocol.
None
Great ELISA kit we have been using for many years. The cost of the ELISA also makes it very affordable if performed routinely.