Histone H3K9me3 Antibody for Western Blot

Central Laboratory
Shin-Kong Wu Ho-Su Memorial Hospital
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

GENETEX

Product Name:

Histone H3K9me3 (Tri-methyl Lys9) antibody

Catalog Number:

GTX121677

This antibody was used to detect Histone H3K9me3 (Tri-methyl Lys9)expression in MG63 cell line by western blot analysis.  It is a good antibody which detects the band of interest.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human osteosarcoma cell line whole cell lysate

Primary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Blocking Agent

WB:5% non-fat dry milk in TBST

Secondary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody perfectly detected Histone H3K9me3 (Tri-methyl Lys9) expression by western blot analysis in human osteosarcoma cell line (MG63).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High sensitivity

The Bad

NA

The Bottom Line

We will continue to use this antibody

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