Histone H3K27me3 Antibody for Western Blot

July 19, 2018

Central Laboratory
Shin-Kong Wu Ho-Su Memorial Hospital
Principal Investigator

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Company:

GENETEX

Product Name:

Histone H3K27me3 (Tri-methyl Lys27) antibody

Catalog Number:

GTX121184

This antibody was used to detect Histone H3K27me3 (Tri-methyl Lys27) expression in MG63 cell line by western blot analysis. It is a highly specific antibody with a single band

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human osteosarcoma cell line whole cell lysate

Primary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Blocking Agent

WB:5% non-fat dry milk in TBST

Secondary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody perfectly detected Histone H3K27me3 (Tri-methyl Lys27) expression by western blot analysis in human osteosarcoma cell line (MG63).

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

High sensitivity

The Bad

NA

The Bottom Line

We will continue to use this antibody

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