P-Glycoprotein Antibody for Western Blot

Central Laboratory
Shin-Kong Wu Ho-Su Memorial Hospital
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

GENETEX

Product Name:

P-Glycoprotein antibody

Catalog Number:

GTX108354

This antibody was used to detect P-Glycoprotein expression in MG63 cell line by western blot analysis. Very sensitive and specific signal for Western blot application.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human osteosarcoma cell line whole cell lysate

Primary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Blocking Agent

WB:5% non-fat dry milk in TBST

Secondary Incubation

1:100 dilution overnight at 4 degree in TBST buffer

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

This antibody perfectly detected P Glycoprotein expression by western blot analysis in human osteosarcoma cell line (MG63).

DOI or PMID #

N/A

Additional Notes

A: MG63 whole cell lysate B: doxorubicin resistant MG63 cell line C: Cisplatin resistant MG63 cell line 8% SDS PAGE

Related Categories

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Summary

The Good

High sensitivity

The Bad

Minor background signals

The Bottom Line

We will continue to use this antibody

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