Good Antibody To Stain Germinal Centers

June 19, 2018

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Rat Anti-Mouse T- and B-Cell Activation Antigen

Catalog Number:

553666

We study terminal differentiated B cells - plasma cells. These cells are also produced in structures called germinal centers. Therefore we stained for the presence of germinal center cells that are GL7+FAS+.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mesenteric lymph nodes of SPF and GF mice

Primary Incubation

GL7 FITC (1/200), anti-CD95 PE-Cy7, anti-CD19 incubate 15min at 4°C

Blocking Agent

Fc block with 2.4G2 30 µg/ml and incubate 15min at 4°C

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Mesenteric lymph nodes of GF mice present with a 10 fold reduction of Germinal Center B cells compare to SPF aged matched mice. Germinal center B cells are GL7+CD95+.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very easy to use

The Bad

Could be cheaper

The Bottom Line

The GL7 antibody is quite good and works well, so is a very good option to stain germinal centers.

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