BD Biosciences
FITC Rat Anti-Mouse T- and B-Cell Activation Antigen
553666
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We study terminal differentiated B cells - plasma cells. These cells are also produced in structures called germinal centers. Therefore we stained for the presence of germinal center cells that are GL7+FAS+.
Flow Cytometry
Mesenteric lymph nodes of SPF and GF mice
GL7 FITC (1/200), anti-CD95 PE-Cy7, anti-CD19 incubate 15min at 4°C
Fc block with 2.4G2 30 µg/ml and incubate 15min at 4°C
None
Flow cytometry
Mesenteric lymph nodes of GF mice present with a 10 fold reduction of Germinal Center B cells compare to SPF aged matched mice. Germinal center B cells are GL7+CD95+.
N/A
Very easy to use
Could be cheaper
The GL7 antibody is quite good and works well, so is a very good option to stain germinal centers.