Qiagen
DNA Hydration Solution
1045702
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The goal of our lab is to understand the effect of HIV infection on various aspects of the immune response both in vitro and in vivo. To study these phenomena in vivo, various transgenic mouse lines are utilized including those transgenic for various human and viral genes that require ongoing genotyping efforts. The extraction of adequate yields on quality DNA requires reliable extraction methods. The Qiagen DNA Hydration Solution has proven to be highly efficient and reliable at reconstituting DNA extracts from mouse tails for our genotyping.
DNA extraction
Mouse tail DNA extracts
Collect tail and extract using isopropanol precipitation and ethanol washes. Reconstitute DNA pellet in appropriate volume of DNA Hydration Solution.
Adjust the volume of hydration solution to have the desired DNA concentration for your downstream applications
Solubilized pure DNA extracts
N/A
Large volumes lasts a long time
None
Great DNA solubilization following extraction