DNA Hydration Solution

Albert Einstein College of Medicine
Pathology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

DNA Hydration Solution

Catalog Number:

1045702

The goal of our lab is to understand the effect of HIV infection on various aspects of the immune response both in vitro and in vivo. To study these phenomena in vivo, various transgenic mouse lines are utilized including those transgenic for various human and viral genes that require ongoing genotyping efforts. The extraction of adequate yields on quality DNA requires reliable extraction methods. The Qiagen DNA Hydration Solution has proven to be highly efficient and reliable at reconstituting DNA extracts from mouse tails for our genotyping.

Experimental Design and Results Summary

Application

DNA extraction

Starting Material

Mouse tail DNA extracts

Protocol Overview

Collect tail and extract using isopropanol precipitation and ethanol washes. Reconstitute DNA pellet in appropriate volume of DNA Hydration Solution.

Tips

Adjust the volume of hydration solution to have the desired DNA concentration for your downstream applications

Results Summary

Solubilized pure DNA extracts

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Large volumes lasts a long time

The Bad

None

The Bottom Line

Great DNA solubilization following extraction

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