CD309 Monoclonal Antibody

June 06, 2018

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD309 (FLK1) Monoclonal Antibody (Avas12a1), PE, eBioscience

Catalog Number:

12-5821-82

This antibody was used to analyze the surface expression of VEGFR2 (CD309, Flk1, KDR) in differentiated mouse embryonic stem cells. This monoclonal anti CD309 PE-conjugated antibody appears specific to a subset of differentiated mESCs toward mesoderm.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Fresh Differentiated mouse Embryonic Stem cells toward mesoderm

Primary Incubation

0.5ug of antibody/ million of cells, 20mins, at 4C in Blocking agent

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Primary antibody conjugated with PE, Flow cytometer detection

Results Summary

This antibody works well on fresh, differentiated mouse Embryonic Stem cells. Specificity for surface marker is seen by comparison to isotype PE-conjugated control antibody. It marks a subset of differentiated mESCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signal in a subpopulation of differentiated mouse embryonic stem cells

The Bad

Staining requires fresh cells (unfixed) for optimal signal

The Bottom Line

Good antibody overall. Recommended for flow cytometry study of mESCs in vitro differentiation. This antibody reacts with CD309/VEGFR2, a cell surface receptor, which marks a subset of early mesodermal derivatives

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