CD4 Magnetic Bead Isolation

Albert Einstein College of Medicine
Pathology
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD4 microbeads, human

Catalog Number:

130-045-101

Our lab studies HIV immunology which requires the isolation of CD4+ cells for in vitro infection. These beads provide a reliable way to isolate this cell population from human PBMCs for downstream applications.

Experimental Design and Results Summary

Application

Cell isolation/positive selection of cells

Starting Material

Human PBMCs

Protocol Overview

Isolate and count cells, resuspend in MACS buffer, incubate with CD4 magnetic beads, run over magnetic column

Tips

Titrate beads to optimize the actual amount of beads needed

Results Summary

Highly enriched CD4+ cells

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy protocol for positive selection of cells

The Bad

None

The Bottom Line

Easy to use protocol for enrichment of CD4+ cells.

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