This Antibody Works Great On Western Blots

Pathology
University of Pittsburgh Medical Center
Research Instructor

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

R&D Systems

Product Name:

Human PD-L1/B7-H1 Antibody

Catalog Number:

AF156-SP

I did a western blot using MDA-MB-231 lysate to compare the anti-PD-L1 antibody from R&D Systems to one from Cell Signaling Technology (Cat. No. #13684S). The dilution of both antibodies is 1:1000. They both produce specific bands with predicted size.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MDA-MB-231 cell lysate

Primary Incubation

1:1000, 4 degrees, Overnight

Blocking Agent

Milk

Secondary Incubation

1:5000, Room temperature, 1h

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Antibody produces specific band with the predicted size. It is comparable to the antibody from Cell Signaling in terms of performance.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specificity

The Bad

Sensitivity

The Bottom Line

I would strongly recommend this product to colleagues.

Share your experience with other scientists. Write a Review! »

Join the discussion