Effective For Distinguishing Live Populations

The Gladstone Institutes
GIVI
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen (Thermo Fisher Scientific)

Product Name:

LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation

Catalog Number:

L34957

I work with tough tissues that require a lot of processing to isolate cells of interest. This processing causes a great deal
of cell death, it becomes vital to distinguish the live populations amongst the pool of dead cells. This kit has been
wonderful at achieving this.

Experimental Design and Results Summary

Application

Fluorescent staining

Starting Material

Isolated human lymphocytes

Protocol Overview

Product is diluted in 50 ul and saved in 5 ul aliquots. 1 ul is used per 500 ul of sample and subsequently stained at room temperature for 15 minutes. Samples are then washed.

Tips

It is advisable to save this product in aliquots as repeated freeze/thaw detrimental to its integrity

Results Summary

Results are great and properly isolates live and dead populations!

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very easy to use!

The Bad

N/A

The Bottom Line

Great product for distinguishing between live and dead cell populations.

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