Great Chambers For Electrophoresis

University of Kansas Medical Center
Pathology
Postdoctoral Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Mini PROTEAN(R) Tetra Cell Electrophoresis Chamber

Catalog Number:

1658004

We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what mechanisms, if any, may lead to resistance to theses ATPase inhibitors. We are measuring changes in protein expression in an animal model of drug resistance. We purchased these systems because of their price, and because we can use precast gels which help decrease variability among the researchers in the lab.

Experimental Design and Results Summary

Application

Electrophoresis (Western blotting)

Starting Material

Tumor cell lysates

Protocol Overview

I run polyacylamide gels for Westerns at 60V for 15 minutes, then increase to 100V until the end of the run.

Tips

Don't damage or bend the wire that carries current during washing and storage! It will affect the eveness of your lanes/wells.

Results Summary

I have had success using this chamber system for 6 years, without requiring any repairs or replacements. As long at the wire isn't damaged or bent, wells/lanes run evenly and I can readily reproduce my data.

Features Summary

Run 4 gels in one chamber!

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Great price, can run 4 gels at once!

The Bad

Have to be cautious with washing and storing.

The Bottom Line

Great product, I really have no complaints at all.

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