Santa Cruz Biotechnology
PARP-1 (F-2) antibody
sc-8007
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: PARP-1 Western Blot Antibodies
This antibody was used in a study where induction of apoptosis in human tumor cells was followed up by detection of cleaved PARP-1 in western blots.
Western Blot
Human tumor cell lysates
Antibody at a final concentration of 1 microgram/ml, ON incubation with gentle rocking in a cold room
5% milk in PBS-0.05% Tween 20
HRP-conjugated anti-mouse antibody at 1:5,000 in 5% milk in PBS-0.05% Tween 20
N/A
West Pico
This mouse monoclonal antibody, raised against PARP-1 C-terminus, detects with high efficiency both the full-length and cleaved PARP-1 in human tumor cell lysates.
The image demonstrates protein bands corresponding to the full-length PARP-1 (~116 kDa) and cleaved PARP-1 (~89 kDa). Confirming high specificity of this antibody, the cleaved PARP-1 is detected only in the cells, which underwent apoptotic induction (right lane).
This antibody is highly sensitive and specific.
None detected
This mouse monoclonal antibody is highly recommended for western blot detection of the full-length and cleaved RAPR-1 in lysates of human tumor cells.