Antibody To Detect Total Akt

April 11, 2018

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technolgoy

Product Name:

Akt (pan) (11E7)

Catalog Number:

4685

This antibody was used in a study that investigated the role of Akt activation (Akt phosphorylation) in tumor cell survival. To validate Akt activation, the levels of pAkt should be compared to total Akt levels. The antibody reviewed here (4685) produced excellent results in western blot analysis of tumor cell lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human tumor cell lysates

Primary Incubation

Antibody at a final concentration of 1 microgram/ml, ON incubation with gentle rocking in a cold room

Blocking Agent

5% BSA in PBS-0.05% Tween; 45-60 min, RT, gentle rocking

Secondary Incubation

Goat anti rabbit IgG conjugated with HRP, 1:3,000; 1 hr; RT; gentle rocking

Tertiary Incubation

N/A

Detection

West Pico

Results Summary

This pan Akt rabbit antibody #4685 works excellently in western blot analysis: The antibody does not produce non-specific bands and can be used directly or after initial pAkt detection.

DOI or PMID #

N/A

Additional Notes

The image demonstrates similar levels of total Akt in the lysates of serum-starved cells that were treated with the Akt-activating agents (FBS or HGF) or left untreated.

Image Gallery

Summary

The Good

Highly specific antibody to detect total Akt in western blotting. The diluted antibody can be frozen and re-used many times.

The Bad

None detected

The Bottom Line

This is a very good antibody to detect total (pan) Akt in the cell samples were activation of Akt should be confirmed by "loading control".

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