Neutrophil Depletion in A Mouse by Injecting 1A8 Antibody

April 05, 2018

Microbiology & Immunology
University of Rochester Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioXcell

Product Name:

1A8 antibody (anti-mouse Ly6G)

Catalog Number:

BE0075-1

Depleting a specific cell type in an experimental animal is extremely useful to investigate the functions specific for the cell. However, it is very challenging, so specific deletion is hardly attainable in most cases. Mouse neutrophil is an exception. Mouse neutrophils express a highly specific cell surface protein, Ly6G, and injection of anti-Ly6G antibody is a well-accepted method to delete neutrophils in a mouse.

Experimental Design and Results Summary

Applications

Neutrophil depletion

Sample

Mouse

Primary Incubation

I.P. injection to a mouse

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

None

Results Summary

Significant depletion of mouse neutrophils can be attained by one-time intraperitoneal injection of 300 - 500 ug of 1A8 antibody (anti-mouse Ly6G antibody) within 48 hours. The amount of antibody may be adjustable according to the mouse body weight and the efficiency may be even heightened by an additional secondary administration (such as 50-80 ug intratracheal administration for the study of airways). In the figure1A attached, neutrophils in spleen marked in dotted rectangle were barely detected in 1A8 injected mouse. In the figure 1B, neutrophils in influenza virus-infected lung and trachea were significantly depleted by 1A8 antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

BioXcell provides reliable antibodies to be used in bulk.

The Bad

The discount is only for non-profit academic usage.

The Bottom Line

Specific depletion of neutrophils in a mouse can be attainable by injecting 1A8 antibody from BioXcell, who provides a variety of monoclonal antibody in bulk of high purity.

Share your experience with other scientists. Write a Review! »

Join the discussion