Biolegend
APC anti-mouse CD279 (PD-1) Antibody
135209
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Some of our research focuses on the study of T cells during and after influenza infection in the mouse model. Since PD1 is an inhibitor of T cell function, we are interested to see how it's expression changes around an infection and also secondary infections in the mouse. We chose this antibody because it was bright and worked easily in our panel.
Flow Cytometry
Mouse lung cells
Cells were stained in 100uL FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100uL FACS buffer.
FACS buffer
None
LSR II
By using this antibody, we are able to see the expression of PD1 by some T cells in our sample and calculate the MFI of those cells in different treatment groups.
N/A
Bright and stable
This antibody works well in different mouse treatment groups and is bright with low background.