Staining Human T Cells With Anti-Ki67 Alexa647

May 04, 2018

Clinical and Translational Research
The Research Institute at Nationwide Children's Hospital
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

anti-human Ki67 Alexa647

Catalog Number:

350509

This antibody was used along with 7-AAD for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used clone for Ki67, Alexa647 is bright and works well with other fluorophores in the staining panel, and Biolegend had a competitive price.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T cells

Primary Incubation

5 ul per test in 100uL total staining volume for 15 minutes at room temperature.

Blocking Agent

2% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSR II

Results Summary

Ki67 staining was excellent. Each stage of the cell cycle was detectable after staining.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well. Low cost

The Bad

N/A

The Bottom Line

Will continue to use this antibody.

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