Biolegend
anti-mouse TNFa APC
506307
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This antibody was used to stain TNFa producing T cells in mouse splenocytes. It was chosen because it is a commonly used clone for TNFa, APC is bright and works well with other fluorophores in the staining panel, and Biolegend had a competitive price.
Flow Cytometry
Mouse splenocytes
1:100 dilution in 100uL total staining volume for 15 minutes at room temperature.
FcBlock + 2% FBS
N/A
BD LSR II
TNFa staining was excellent. Positive cells were fully resolved from negative.
Works well. Low cost
Will continue to use this antibody.