MMRN2 Antibody for Western Blot of HUVEC Lysates

Biological Sciences Platform
Sunnybrook Research Institute

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Company:

Abnova

Product Name:

MMRN2 purified MaxPab mouse polyclonal antibody (B01P)

Catalog Number:

H00079812-B01P

The mouse polyclonal antibody was used for western blot analysis of HUVEC lysates that had been transfected with two siRNA duplexes against MMRN2 revealing reduction in the protein bands being picked up by the MMRN2 antibody in comparison to a negative control duplex (NCD).

Experimental Design and Results Summary

Applications

Western Blot

Sample

HUVEC lysates

Primary Incubation

1 hour at room temperature or overnight 4 degrees, 2ug/mL

Blocking Agent

3% BSA PBST

Secondary Incubation

Anti-rabbit HRP 1:5000, 1 room temperature

Tertiary Incubation

None

Detection

Enhanced chemiluminescence

Results Summary

The mouse polyclonal anitbody was used for western blot analysis of HUVEC lysates that had been transfected with two siRNA duplexes against MMRN2 revealing reduction in the protein bands being picked up by the MMRN2 antibody in comparison to a negative control duplex (NCD). The antibody picked up two bands between 130-250 kDa under reducing conditions, which may correspond to differentially glycosylated versions of MMRN2 or proteolytically cleaved versions. The antibody is specific and sensitive.

DOI or PMID #

28671670

Additional Notes

N/A

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Summary

The Good

Highly specific

The Bad

Expensive

The Bottom Line

A good antibody for western blotting of human endothelial cells

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